WITH EPICENTRE KIT + GLASS BEADS (Pathogen Lysis Tubes QIAGEN)
1. Centrifuge dental plaque sample @ 12,000 rpm 5 min, discard supernatant
2. Add 500 ul of (1X) Lysis buffer. Vortex at max speed 5 min
3. Add the mix to a Pathogen Lysis Tube L (Ref # 19092 QIAGEN)
4. Place it on a TissueLyzer LT and disrupt cells for 10 min @ 50 Hz
5. Remove the tube from the tissuelyzer and incubate 3 min @ RT to reduce the foam in the tube
6. Add 50 ul lysozyme 20 mg/ml and Incubate 30 min @ 37 C with agitation
7. Add 2 ul proteinase K and incubate 15 min @ 65 C with agitation
8. Place on ice 3 min
9. Add 300 Protein Precipitation Reagent and vortex vigorously 10 min
10. Centrifuge 10 min @ 4 C
11. Discard pellet, KEEP supernatant containing your nucleic Acids
